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胰岛内分泌细胞弥散地分布在大量胰腺外分泌腺泡组织之间,因此一度曾使胰腺内分泌研究受到限制。体外单层细胞培养法是研究胰岛细胞生理、生化及形态学的一个十分有用的工具。自1967年Lacy等采用胶原酶消化法分离胰岛获得成功以来,才使胰岛细胞离体培养成为可能。但在胰岛细胞离体培养中仍存在着克服成纤维细胞过度生长的难题。本文采用新生大鼠的胰岛细胞,经胶原酶反复消化法分离,应用接种后18小时更换培养瓶,以及在培养瓶内壁涂以鼠尾胶等方法,使成纤维细胞明显减少,胰岛细胞贴瓶率提高,在体外连续
Islet endocrine cells diffusely distributed between a large number of pancreatic exocrine acinar tissue, so once the pancreatic endocrine research has been limited. In vitro monolayer cell culture is a very useful tool for studying islet cell physiology, biochemistry and morphology. Since 1967, Lacy and other collagenase digestion method used to isolate islets successfully, it is possible to culture islet cells in vitro. However, in vitro culture of islet cells there is still the problem of overcoming the excessive growth of fibroblasts. In this paper, neonatal rat islet cells were separated by repeated digestion of collagenase, the application of 18 hours after inoculation of the flask, and the inner wall of the flask coated with rat tail glue and other methods to significantly reduce fibroblasts, islet cells labeled Increased rate, continuous in vitro