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目的:建立大鼠肺纤维化动物模型,探讨甘利欣治疗博莱霉素致肺纤维化大鼠的作用机制。方法:将大鼠40只,随机分成4组:博莱霉素所致大鼠肺纤维化模型组(BLM组)、生理盐水对照组、甘利欣治疗组、地塞米松(dexamethasone,DXM)治疗组。经实验后观察各组大鼠免疫组化TGF-β1、IFN-γ表达观察;HE染色病理观察及测定各组大鼠血清TGF-β1IL-4、IFN-γ含量。结果:①甘利欣治疗组大鼠肺纤维化程度较轻微;②模型组大鼠肺组织TGF-β1染色呈强阳性反应,IFN-γ染色较淡,而甘利欣治疗组TGF-β1、IFN-γ染色则相反。免疫组化TGF-β1甘利欣组与地塞米松组相比,P为0.018。免疫组化IFN-γ甘利欣组与地塞米松组相比,P为0.618;③模型组大鼠血清TGF-β1、IL-4含量较高,IFN-γ含量较低,而甘利欣治疗组、DXM治疗组则相反。大鼠血清TGF-β1地塞米松组与甘利欣组相比P为0.169;大鼠血清IL-4地塞米松组、甘利欣组相比无差异;地塞米松组和甘利欣组IFN-γ均数相比也无差异(P>0.05),但两组与模型组相比P分别为0.001,0.000。结论:甘利欣可以减轻大鼠肺纤维化损伤和肺组织的TGF-β1、IL-4的表达,并降低他们在血清中的含量,增加血清中的IFN-γ含量,增强肺组织IFN-γ的表达。
OBJECTIVE: To establish an animal model of pulmonary fibrosis in rats and explore the mechanism of action of Glycyrrhizin in bleomycin-induced pulmonary fibrosis in rats. Methods: Forty rats were randomly divided into four groups: bleomycin-induced pulmonary fibrosis model group (BLM group), normal saline control group, glycyrrhizin treatment group, dexamethasone (DXM) treatment group. After the experiment, the expression of TGF-β1 and IFN-γ in each group were observed; the contents of TGF-β1 IL-4 and IFN-γ in the serum of each group were observed by HE staining. Results: ① The degree of pulmonary fibrosis in rats treated with Glycyrrhizin was milder; ② The positive staining of TGF-β1 in the lung tissue of model rats was strongly positive, the staining of IFN-γ was lighter, while the expressions of TGF-β1 and IFN- The opposite of gamma staining. Immunohistochemical TGF-β1 Glycyrrhizin group and dexamethasone group, P 0.018. Compared with dexamethasone group, the P value of immunohistochemical IFN-γ-Glycyrrhizin group was 0.618; ③The serum levels of TGF-β1 and IL-4 in model group were higher and the content of IFN-γ was lower, , DXM treatment group is the opposite. The serum level of TGF-β1 in dexamethasone group and the Glycyrrhiza group was 0.169; there was no difference in serum IL-4 dexamethasone group and Glycyrrhiza group; the levels of IFN-γ in dexamethasone group and the Glycyrrhiza group There was no difference between the two groups (P> 0.05), but the P values of the two groups were 0.001 and 0.000 respectively. Conclusion: Glycyrrhizin can attenuate pulmonary fibrosis in rats and the expression of TGF-β1 and IL-4 in lung tissue, decrease their content in serum, increase the content of IFN-γ in serum, and enhance the expression of IFN-γ expression.