大花蕙兰原球茎诱导、增殖与分化影响因素研究

• 作 者: 徐萌 郭绍霞 孙丽 张颖
• 机 构: 青岛农业大学园林与林学院,
• 出 处: 北方园艺
• 发表日期: 2016年16期
• 关键词: 原球茎诱导 大花蕙兰 原球茎 诱导 增殖 原球茎增殖 活性炭 不定芽 基本培养基 培养基

以大花蕙兰试管组织为试材,MS为基本培养基,研究了不同添加物6-BA、NAA、IBA、活性炭,对大花蕙兰原球茎诱导、增殖和分化的影响。结果表明:适宜原球茎诱导、增殖的最佳培养基为MS+1.00mg·L~(-1) 6-BA+0.1mg·L~(-1) NAA,适宜原球茎分化的最佳培养基为MS+1.0mg·L~(-1) 6-BA+0.2mg·L~(-1) NAA,在培养基中添加0.3%活性炭有利于原球茎的增殖和分化。

Cymbidium as the test material, MS as the basic medium, studied the different additives 6-BA, NAA, IBA, activated carbon, Cymbidium protocorm induction, proliferation and differentiation. The results showed that the best culture medium for the induction and propagation of protocorm was MS + 1.00mg · L -1 (-1) 6-BA + 0.1mg · L -1 NAA MS + 1.0mg · L -1 (-1) 6-BA + 0.2mg · L -1 NAA, the addition of 0.3% activated carbon in the medium was good for the proliferation and differentiation of protocorms.