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目的研究大鼠孕期暴露于苯乙基异硫氰酸盐(PEITC)对子一代(F1)神经发育、早期行为及学习记忆能力等的影响。方法受孕大鼠于孕7～16天分别经口给予0、15、60和120mg/kg PEITC后,观察F1代断崖回避、平面翻正、前肢悬挂、听觉惊愕和嗅觉定位等早期行为发育指标的变化,并应用Y迷宫评价仔鼠学习记忆能力,对F1代大鼠大脑海马区神经元发育情况进行病理学检查。结果孕大鼠60和120mg/kg染毒剂量组,其F1代仔鼠平面翻正、断崖回避以及嗅觉定向等早期行为指标发育延迟,且运动学习能力下降;120mg/kg染毒剂量组F1代仔鼠海马区平均神经元密度为142±6.7,与阴性对照组相比有显著差异(P<0.05),且神经元损伤评分增至0～1级。结论大鼠孕期给予PEITC,可对F1代大鼠早期行为和学习记忆产生抑制和干扰;PEITC在15mg/kg剂量条件下无神经发育毒性和行为致畸作用。
Objective To investigate the effects of exposure to phenylethyl isothiocyanate (PGEc) during pregnancy on the neurodevelopment, early behavior, learning and memory abilities of F1 generation in rats. Methods The pregnant rats were orally administered 0, 15, 60 and 120 mg / kg PEITC respectively from 7 to 16 days of gestation. The early behavioral development indexes such as avoidance of bluffing, plane correction, forelimb suspension, auditory discomfort and olfactory orientation were observed The Y maze was used to evaluate the ability of learning and memory in the offspring. Pathological examination was performed on the neuronal development in the hippocampus of F1 generation rats. Results At the dose of 60 and 120 mg / kg in pregnant rats, the early behavioral indexes such as plane inversion, cliff avoidance and olfaction orientation were delayed and the ability of motor learning was decreased. The F1 generation of 120 mg / kg dose group The mean neuronal density in the hippocampus of offspring rats was 142 ± 6.7, which was significantly different from that in the negative control group (P <0.05). The neuronal injury score increased to 0 ~ 1 level. Conclusion PEITC during pregnancy may inhibit the early behavior and learning and memory of F1 rats. PEITC has no neurotoxicity and behavioral teratogenicity at 15 mg / kg.