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目的:研究金黄色葡萄球菌α-溶血素(α-hemolysin,Hla)突变体H35A在人外周血单个核细胞(human peripheral blood mononuclear cells,h PBMC)和THP-1巨噬细胞中对于野生型Hla毒性作用的影响。方法:H35A与Hla共同作用于h PBMC和THP-1巨噬细胞,实时荧光定量PCR检测细胞中白介素(interleukin,IL)-1β、IL-6、肿瘤坏死因子(tumor necrosis factor,TNF)-α等炎症因子的m RNA转录水平差异,台盼蓝染色计算THP-1巨噬细胞存活率。结果:预孵育突变体H35A 1 h的h PBMC和THP-1巨噬细胞,经Hla刺激产生IL-1、IL-6、TNF-α的m RNA转录水平显著降低;台盼蓝染色计数发现,H35A与Hla共同作用的THP-1巨噬细胞存活率显著高于Hla单独处理组。结论:突变体H35A可抑制野生型Hla对于h PBMC和巨噬细胞的毒性作用,为金黄色葡萄球菌早期感染防治药物的研发提供了更多选择。
Objective: To investigate the effect of mutant H35A of Staphylococcus aureus on human peripheral blood mononuclear cells (h PBMCs) and THP-1 macrophages Effects of toxic effects. Methods: H35A and Hla co-administered h PBMCs and THP-1 macrophages. The levels of interleukin (IL) -1β, IL-6 and tumor necrosis factor (TNF) -α And other inflammatory cytokines m RNA transcription level differences, trypan blue staining THP-1 macrophage survival. Results: The mRNA levels of m RNA in IL-1, IL-6 and TNF-α induced by Hla stimulation were significantly decreased in h PBMCs and THP-1 macrophages pretreated with mutant H35A for 1 h. The results of trypan blue staining showed that, The survival rate of THP-1 macrophages that H35A interacted with Hla was significantly higher than that of Hla alone. Conclusion: The mutant H35A can inhibit the virulence of wild-type Hla on h PBMCs and macrophages and provide more options for the development and prevention of S. aureus infection.