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目的:了解2009年兰州地区5岁以下婴幼儿轮状病毒(Rotavirus,RV)腹泻流行状况及毒株变异特点,为疫苗的研发提供流行病学资料。方法:在哨点监测医院(兰州大学第一医院)收集5岁以下住院患儿粪便标本313份,采用ELISA检测RV,阳性标本用RT-PCR进行G、P分型。结果:2009年RV检出率为50.80%(159/313),腹泻高发季节在10～12月。G分型中以G3型(51.57%)为主,其次是G1(7.55%)、G2(6.29%)和G9(5.66%)。P分型中以P[8]型(24.14%)为主。G-P组合主要以P[8]G3型(33.82%)、P[4]G3型(19.12%)、P[4]G2型(5.88%)和P[8]G1型(1.47%)为主,另外还发现P[6]G3型、P[6]G9型、P[4]G9型、P[8]G9型等较少见的组合型。结论:RV是引起兰州地区婴幼儿腹泻最主要的病原,毒株呈现型的多样性变化。
OBJECTIVE: To understand the epidemic situation of rotavirus (RV) diarrhea and the variation of strains in Lanzhou area in 2009 and to provide epidemiological data for the development of the vaccine. Methods: 313 stool samples from hospitalized children under 5 years of age were collected at the sentinel monitoring hospital (Lanzhou University First Hospital). RV was detected by ELISA. The positive and negative samples were genotyped by RT-PCR. Results: The detection rate of RV in 2009 was 50.80% (159/313). The incidence of diarrhea was in the period of 10-12 months. G genotype G3 (51.57%), followed by G1 (7.55%), G2 (6.29%) and G9 (5.66%). P type in the P [8] type (24.14%) based. GP combinations were mainly dominated by P [8] G3 (33.82%), P [4] G3 (19.12%), P [4] G2 (5.88%) and P [8] G1 (1.47% In addition, rare combinations of P [6] G3, P [6] G9, P [4] G9, P [8] G9 and the like were also found. Conclusion: RV is the most important cause of infant diarrhea in Lanzhou.