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目的 探讨血管内皮生长因子 (VEGF)及其受体在肝癌细胞系中的表达及意义 .方法 采用免疫组织化学及免疫荧光方法检测了两株肝癌细胞株及一株正常肝细胞株中VEGF及其受体的表达 ,并用 Western blot法对 VEGF表达半定量 .结果 VEGF在肝癌细胞株 SMMC 772 1及 HHCC中以及正常肝细胞株 QZG中均有表达 ,灰度值分别为 2 18±4,2 15± 4和 2 2 8± 6 ,SMMC 772 1及 HHCC中 VEGF表达强于 QZG,两者之间有显著统计学差异 (P<0 .0 5 ) ;VEGF受体KDR在 HHCC中呈弱阳性表达 ,在 772 1及 QZG中无表达 ,受体 flt- 1在三株细胞中均无表达 .结论 VEGF及其受体在肝癌细胞中的表达与肝细胞癌的发生发展有关
Objective To investigate the expression and significance of vascular endothelial growth factor (VEGF) and its receptor in hepatoma cell lines. Methods Immunohistochemical and immunofluorescence methods were used to detect VEGF in two hepatoma cell lines and one normal liver cell line. Expression of the receptors and semi-quantitation of VEGF expression by Western blotting. Results VEGF was expressed in hepatoma cell lines SMMC 772 1 and HHCC and in normal hepatocyte cell lines QZG with gray values of 2 18±4, 2 15 respectively. The expression of VEGF in SMMC 7721 and HHCC was higher than that of QZG in ± 4 and 268±6. There was a significant difference between them (P<0.05). The expression of VEGF receptor KDR was weakly positive in HHCC. There was no expression in 7721 and QZG, and the receptor flt-1 was not expressed in all three cells. Conclusion The expression of VEGF and its receptor in hepatocellular carcinoma is related to the development of hepatocellular carcinoma.