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目的探讨胃肠引流液中幽门螺杆菌(Helicobacterpylori,H.pylori)的检出率及患者VitC水平。方法用PCR技术、细菌培养、快速尿素酶试验检测胃肠引流液中的H.pylori,用反相液相色谱法检测患者胃肠引流液及血浆中VitC含量。结果282例患者胃肠引流液中H.pylori检出率为41.1%(116/282),PCR、细菌培养及快速尿素酶试验阳性率分别为42.9%(121/282)、11.3%(32/282)、41.1%(116/282)。胃溃疡、十二指肠溃疡、复合溃疡、胃癌、急性肠梗阻、急性胆囊炎及门静脉高压合并食管胃底静脉曲张患者H.pylori检出率分别为54.6%(30/55)、52.2%(24/46)、58.3%(7/12)、48.2%(27/56)、22.2%(8/36)、27.6%(16/58)、21.1%(4/19),差异具显著性(P<0.01)。胃溃疡、十二指肠溃疡、复合溃疡、胃癌H.pylori阳性患者与H.pylori阴性患者血浆及胃肠引流液VitC浓度相比,差异均具有显著性(P<0.01)。结论胃肠引流液中可检出H.pylori,且能分离培养出活菌;PCR技术是一种敏感、有效的检测胃引流液中H.pylori的方法。H.pylori感染人体后引起胃肠引流液及血浆中VitC含量的减少,可能与胃十二指肠疾病的发生有关。
Objective To investigate the detection rate of Helicobacter pylori (Helicobacter pylori) and the level of VitC in patients with gastrointestinal drainage. Methods H.pylori in gastrointestinal drainage fluid was detected by PCR, bacterial culture and rapid urease test. The content of VitC in gastrointestinal fluid and plasma was detected by reverse phase liquid chromatography. Results The positive rate of H.pylori in gastrointestinal drainage fluid was 41.1% (116/282) in 282 patients. The positive rates of PCR, bacterial culture and rapid urease test were 42.9% (121/282) and 11.3% (32 / 282), 41.1% (116/282). The detection rates of H.pylori in gastric ulcer, duodenal ulcer, complex ulcer, gastric cancer, acute intestinal obstruction, acute cholecystitis and portal hypertension combined with esophagogastric varices were 54.6% (30/55) and 52.2% The difference was statistically significant (P <0.05). The difference was significant (P <0.05) P <0.01). Gastric ulcer, duodenal ulcer, complex ulcer, gastric H.pylori positive patients and H.pylori negative patients with plasma and gastrointestinal fluid VitC concentration, the difference was significant (P <0.01). Conclusions H.pylori can be detected in gastrointestinal drainage fluid and live bacteria can be isolated and cultured. PCR technique is a sensitive and effective method to detect H.pylori in gastric drainage fluid. H.pylori infection in the human body caused by gastrointestinal drainage fluid and plasma vitamin C levels decreased may be related to the occurrence of gastroduodenal diseases.