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用苯浸提—甲醇沉淀法, 从杜仲愈伤组织干粉中提取杜仲胶。杜仲胶的平均得率为2.45% 。比较了不同浸提时间和不同浸提温度对胶产量的影响。24~28h 是最适宜的浸提时间,28~35℃最适宜于胶的提取。经红外光谱和紫外光谱检测,证明愈伤组织中的杜仲胶与原植株中的相同。
Using benzene extraction-methanol precipitation method, eucommia gum was extracted from the dried callus of eucommia bark callus. The average yield of gutta percha is 2.45%. The effect of different extraction time and different extraction temperature on gum yield was compared. 24~28h is the most suitable extraction time, 28~35°C is most suitable for the extraction of gum. The FTIR and UV spectra showed that the gutta percha in the callus was the same as that in the original plant.