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目的探讨清胰颗粒(Qingyi Granule,QYG)对重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠肝、肾组织中高迁移率族通道蛋白1(high mobility group box-1,HMGB1)表达的影响。方法54只SD大鼠按随机数字表分为假手术(sham operation,SO)组、SAP组和QYG组;SAP动物模型采用5%牛黄胆酸钠(sodium taurocholate,STC)诱导;苏木精和伊红(hematoxylin and eosin,HE)染色评价3组大鼠肝、肾病理损伤,ELISA法测血清淀粉酶(amylase,AMS)、丙二醛(MDA)、白细胞介素-1(IL-1)和HMGB1水平,免疫组化检测肝、肾组织中HMGB1蛋白表达;逆转录-聚合酶链式反应(RTPCR)检测肝、肾组织HMGB1 mRNA表达。结果 SAP组大鼠肝、肾组织病理评分、血清AMS、MDA、IL-1和HMGB1水平和肝、肾组织HMGB1蛋白及mRNA表达均较SO组明显升高(P<0.05,P<0.01);QYG能有效下调上述各指标血清水平和肝、肾组织HMGB1蛋白及mRNA表达量,治疗72 h疗效最显著(P<0.05,P<0.01),并呈时效依赖性。结论 HMGB1参与SAP并发肝、肾组织损伤过程,QYG可有效抑制HMGB1表达,从而减轻SAP时肝、肾组织损伤。
Objective To investigate the effect of Qingyi Granule (QYG) on the expression of high mobility group box-1 (HMGB1) in liver and kidney of rats with severe acute pancreatitis (SAP). Methods Fifty-four SD rats were randomly divided into sham operation (SO) group, SAP group and QYG group. SAP animal model was induced by 5% sodium taurocholate (STC) The hematoxylin and eosin (HE) staining was used to evaluate the pathological changes of liver and kidney in rats in the three groups. The levels of amylase (AMS), malondialdehyde (MDA), interleukin-1 (IL- HMGB1 and HMGB1 were detected by immunohistochemistry. The expression of HMGB1 in liver and kidney tissues was detected by immunohistochemistry. The expression of HMGB1 mRNA in liver and kidney tissues was detected by reverse transcriptase-polymerase chain reaction (RTPCR). Results The pathological scores of liver and kidney, serum levels of AMS, MDA, IL - 1 and HMGB1, HMGB1 protein and mRNA in liver and kidney of SAP group were significantly higher than those in SO group (P <0.05, P <0.01). QYG can effectively down-regulate serum HMGB1 protein and mRNA levels in each of the above indexes, and has the most significant effect at 72 h (P <0.05, P <0.01), and is time-dependent. Conclusion HMGB1 is involved in the process of liver and kidney injury in SAP. QYG can effectively inhibit the expression of HMGB1, thereby reducing the liver and kidney injury during SAP.