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目的研究替勃龙对缺血性脑损害的神经保护作用及可能机制。方法 30只雌性成年SD大鼠,随机分为假手术组,模型组,替勃龙组;采用大鼠大脑中动脉闭塞制成局灶性脑缺血模型。在缺血2h、再灌注24h后立即断头取脑,应用TTC染色观察脑梗死体积,TUNEL法检测凋亡细胞数,免疫组化法检测Bcl-2以及白介素(IL)-1β和肿瘤坏死因子(TNF)α-的表达,并进行组织病理学观察。结果替勃龙组较模型组脑梗死体积显著缩小(P<0.05);TUNEL阳性细胞数少(P<0.01);Bcl-2阳性细胞增多(P<0.01);IL-1β及TNFα-表达减弱,差异有显著性(P<0.01)。结论替勃龙可减轻去卵巢大鼠脑缺血再灌注损伤,具有神经保护作用。
Objective To study the neuroprotective effect of tibolone on ischemic brain damage and its possible mechanism. Methods Thirty female adult Sprague-Dawley rats were randomly divided into sham-operation group, model group and tibolone group. The model of focal cerebral ischemia was established by middle cerebral artery occlusion in rats. The rats were sacrificed at 2h after ischemia and 24h after reperfusion. The volume of cerebral infarction was detected by TTC staining. The number of apoptotic cells was detected by TUNEL. The expressions of Bcl-2, interleukin (IL) -1β and tumor necrosis factor (TNF) alpha expression, and histopathological observation. Results The volume of cerebral infarction in tibolone group was significantly reduced (P <0.05), the number of TUNEL-positive cells was less (P <0.01), the number of Bcl-2 positive cells was increased (P <0.01) , The difference was significant (P <0.01). Conclusion Tibolone can attenuate cerebral ischemia-reperfusion injury in ovariectomized rats and has neuroprotective effect.