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用混合单克隆抗体双抗体夹心-ELISA法检测19例痰检虫卵阳性肺吸虫病人,血清中循环抗原全部(100％)皆呈阳性反应。59例根据临床和实验室证据(三项以上免疫学试验呈阳性反应)确诊者,56例(94.9％)显示阳性。在治疗后6个月-3年患者的15例中,14例(93.3％)转为阴性,另一例仍呈阳性反应,因系采用硫双二氯酚治疗未完成疗程者。89名正常人和109例其它7种寄生虫病人,全部皆为阴性,本试验未出现任何假阳性和交叉反应。
Nineteen cases of sputum test-positive egg-paragonimiasis were detected by mixed monoclonal antibody double-antibody sandwich-ELISA. All the circulating antigens in serum were positive (100%). Fifty-nine patients were diagnosed based on clinical and laboratory evidence of a positive response to three or more immunological tests, and 56 (94.9%) showed positive results. Of the 15 patients 6 months to 3 years after treatment, 14 (93.3%) became negative and the other was still positive because of the use of sulfurdodichlorophenol in patients who did not complete the course of treatment. 89 normal subjects and 109 other 7 parasite patients, all of which were negative. No false positives or cross-reactivity were observed in this test.