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目的：为研究受精卵早期发育机理提供大量、同步分裂的受精卵；寻找一种更好的受精卵体外培养基。方法：小白鼠超排取卵，附睾取精，建立体外受精模型。并比较不同培养液对受精卵发育的影响。结论：建立了昆明系小鼠的体外受精模型。受精２４ｈ后，Ｍ１６和ＫＳＯＭ分别有３１．５％和４８．７％发育到２－细胞期；受精４８ｈ后，则分别有１５．７％和５３．６％发育到４－细胞期。结论：ＫＳＯＭ在一定程度上克服“二细胞阻滞”
OBJECTIVE: To provide a large number of synchronous zygotes for studying the mechanism of early development of fertilized eggs, and to find out a better zygote culture medium in vitro. Methods: The mice were ovariectomized, the epididymis was taken, and the in vitro fertilization model was established. The effects of different culture media on the development of fertilized eggs were compared. Conclusion: In vitro fertilization model of Kunming mice was established. After fertilization for 24 h, 31.5% and 48.7% of M16 and KSOM developed into 2-cell stage, respectively. After fertilization for 48h, 15.7% and 53.6% of M16 and KSOM developed into 4-cell stage respectively. Conclusion: KSOM to some extent overcome the “two cell block”