论文部分内容阅读
应用Ｌａｄｉｓｃｈ分部及微量分析方法，对正常早孕和服用三苯氧胺（４０ｍｇ／天，连用４天）早孕妇女的子宫蜕膜鞘糖脂进行定性和定量分析比较。结果表明三苯氧胺组的中性鞘糖脂（Ｎ－ＧＳＬ）总量是正常早孕组的１．８１倍（Ｐ＜０．０１）。高效薄板层析（ＨＰＴＬＣ）分析其图谱主要成分为单己糖基神经酰胺（ＣＭＨ）、双己糖基神经酰胺（ＣＤＨ）、三己糖基神经酰胺（ＣＴＨ）、红细胞糖苷脂（Ｇｌｏｂ）和副红细胞糖苷脂（ＰＧ）。其中ＣＭＨ与ＣＤＨ分别是正常早孕组的２．９８倍（Ｐ＜０．０１）与２．３６倍（Ｐ＜０．０５）。神经节苷脂（Ｇｇ）总量及其组分的ＨＰＴＬＣ定性和定量分析在三苯氧胺组与正常早孕组之间无显著性差异（Ｐ＞０．０５）。提示三苯氧胺主要引起蜕膜Ｎ－ＧＳＬ升高，而对Ｇｇ无明显影响，从而使Ｎ－ＧＳＬ／Ｇｇ比例发生变化，这些变化可能与其抗早孕作用有关。
The use of Ladisch Division and microanalysis methods to qualitative and quantitative analysis of uterine decidual sphingolipids in normal early pregnancy and taking tamoxifen (40mg / day for 4 days) women. The results showed that the total amount of neutral glycosphingolipid (N-GSL) in the tamoxifen group was 1.81 times that of the normal pregnancy group (P <0.01). High performance thin layer chromatography (HPTLC) analysis of its main components of the spectrum of mono-hexosylceramide (CMH), hexanosyl ceramide (CDH), three hexosyl ceramide (CTH), red blood cell glycosides (Glob) and Paraglucinoside (PG). Among them, CMH and CDH were 2.98-fold (P <0.01) and 2.36-fold (P <0.05) higher than normal pregnancy group respectively. Qualitative and quantitative analysis of ganglioside (Gg) total amount and its components by HPTLC showed no significant difference between tamoxifen group and normal early pregnancy group (P> 0.05). Tip tamoxifen mainly caused decidual N-GSL increased, but no significant effect on Gg, so that the ratio of N-GSL / Gg changes, these changes may be related to its role in anti-early pregnancy.