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目的：构建人源性 Ｆａｂ 噬菌体抗体基因库并筛选可表达与肾综合征出血热（ Ｈ Ｆ Ｒ Ｓ）病毒抗原特异结合之 Ｆａｂ 抗体的重组克隆． 方法：利用逆转录、聚合酶链反应（ Ｐ Ｃ Ｒ）等技术从 Ｈ Ｆ Ｒ Ｓ恢复期患者外周血淋巴细胞（ Ｐ Ｂ Ｌ）中扩增出人 Ｉｇ Ｇ抗体重链 Ｆｄ 基因及κ轻链基因，将之克隆入噬菌体载体ｐ Ｃｏｍ ｂ３，构建人源性 Ｆａｂ 噬菌体抗体基因库． 并利用噬菌体表面呈现技术，对此抗体库进行淘筛、富集． 结果：①成功地构建了人源性 Ｆａｂ 抗体基因库，库容量为 ３×１０６ ． ②从噬菌体抗体库中淘筛得到了能表达人源性抗 Ｈ Ｆ Ｒ Ｓ病毒 Ｆａｂ 抗体的重组克隆． 结论：利用噬菌体抗体库技术，可以获得能表达人源性抗 Ｈ Ｆ Ｒ Ｓ病毒抗体的重组克隆．
OBJECTIVE: To construct a human Fab phage antibody library and to screen for recombinant clones expressing Fab antibodies that specifically bind to HFRSV antigens. Methods: Reverse transcription and polymerase chain reaction (PCR) were used to amplify human Ig G antibody heavy chain Fd gene and kappa light chain from peripheral blood lymphocytes (PBL) The gene was cloned into the phage vector p Com b3 to construct a human Fab phage antibody gene library. And the use of phage surface rendering technology, the antibody library Amoy screen, enrichment. Results: ①Human-derived Fab antibody gene library was successfully constructed with a capacity of 3 × 106. ② The recombinant clones expressing human anti-H F R S virus Fab antibody were screened from the phage antibody library. CONCLUSION: Recombinant clones capable of expressing human anti-H F R S virus antibodies can be obtained using phage antibody library technology.