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目的:观察水飞蓟宾减轻庆大霉素引起的肾脏损伤的作用。方法:采用雄性豚鼠50只,随机分为空白对照、庆大霉素和水飞蓟宾低剂量组,水飞蓟宾高剂量组。连续给药10天后,ILISA检测肾组织中MDA(脂质过氧化物),SOD(超氧化物歧化酶),GSH-Px(谷胱甘肽过氧化物酶)和CAT(过氧化氢酶)含量。用透射电镜检测肾脏近曲小管的超微结构。结果:水飞蓟宾(50 mg/kg,100mg/kg)能够显著诱导肾脏细胞内抗氧化酶GSH-Px、CAT和SOD的生成,同时降低MDA的含量。电镜结果显示水飞蓟宾高剂量(100 mg/kg)组明显改善肾小管超微结构。结论:水飞蓟宾可诱导肾脏细胞内抗氧化酶的生成,减少庆大霉素产生的活性氧,进而减轻庆大霉素引起的肾脏毒性。
Objective: To observe the effect of silibinin on gentamicin-induced kidney damage. Methods: Fifty male guinea pigs were randomly divided into blank control group, gentamicin and silybin low-dose group and silybin high-dose group. After 10 days of continuous administration, IL-1β was detected in renal tissue by measuring MDA, SOD, GSH-Px and CAT (catalase) content. Transmission electron microscopy was used to detect the ultrastructure of renal proximal tubule. Results: Silybin (50 mg / kg, 100 mg / kg) significantly induced the generation of antioxidant enzymes GSH-Px, CAT and SOD in the kidney and decreased the content of MDA. Electron microscopy results showed that silibinin at high dose (100 mg / kg) significantly improved renal tubular ultrastructure. Conclusion: Silybin can induce the generation of antioxidant enzymes in the kidney cells, reduce the reactive oxygen species generated by gentamicin, and then reduce the toxicity caused by gentamicin.