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目的研究基于巯基化透明质酸骨架片的牛血清白蛋白的体外释放特性。方法以牛血清白蛋白(BSA)为模型药物,通过测定不同巯基取代度、不同p H下骨架片体外释放度,并采用零级动力学方程、一级动力学方程、Higuchi方程、RitgerPeppas方程、威布尔方程拟合,阐明BSA体外释放的机理。结果巯基化透明质酸能延缓BSA的释放,并随着结合物巯基含量的增加,释放速率减慢;巯基化透明质酸骨架片在p H6.8磷酸盐缓冲液中的释放速率大于在p H1.7氯化钾-盐酸缓冲液中的释放速率。结论基于巯基化透明质酸骨架片的BSA的体外释放机制为骨架溶蚀。
Objective To study the in vitro release characteristics of bovine serum albumin based on thiolated hyaluronic acid matrix tablets. Methods Bovine serum albumin (BSA) was used as a model drug to determine the in vitro release of matrix tablets with different degrees of thiol substitution and different p H values. The kinetic equation, first order kinetic equation, Higuchi equation, Ritger Peppas equation, Weibull equation fitting to elucidate the mechanism of BSA release in vitro. Results Thiolated hyaluronic acid delayed the release of BSA, and the release rate decreased with the increase of sulfhydryl content of the conjugate. The release rate of thiolated hyaluronic acid matrix fragment in p H6.8 phosphate buffer was higher than that in p H1.7 Release rate in potassium chloride-hydrochloric acid buffer. Conclusion The in vitro release mechanism of BSA based on thiolated hyaluronic acid matrix tablets is skeleton dissolution.