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目的 探讨抗氧化剂茶多酚 (teapolyphenols,TP)对环孢菌素A(cyclosporineA ,CsA)诱导慢性肾毒性时细胞凋亡的影响。 方法 采用大鼠CsA慢性肾毒性动物模型 ,分CsA溶剂oliveoil组(n =6)、TP组 (n=6)、CsA组 (n =8)与CsA +TP组 (n =8) ,处理 4周后观察肾功能与组织学改变 ,TUNEL法检测肾细胞凋亡 ,RT PCR检测肾组织caspase 3mRNA的表达 ,并测定caspase 3酶活性。结果 与CsA组相比较 ,CsA与TP联用显著改善CsA诱导的肾功能损伤与组织学改变 ;CsA与TP合用组肾小管及间质凋亡细胞数明显少于CsA组 (7 7± 1 4vs 1 8 9± 3 3 ,P <0 0 5) ,并明显减少CsA诱导的caspase 3mRNA的表达及其酶活性 (P <0 0 5)。 结论 抗氧化剂茶多酚具有抑制CsA诱导慢性肾毒性时肾小管和间质细胞凋亡的作用 ,提示减少CsA诱导的细胞凋亡是茶多酚保护肾功能与组织结构的机理之一。
Objective To investigate the effects of antioxidant teapolyphenols (TP) on the apoptosis of chronic renal nephrotoxicity induced by cyclosporine A (CsA). Methods CsA (n = 8) and CsA + TP group (n = 8) were treated with CsA solvent oliveoil group (n = 6), TP group A week later, renal function and histological changes were observed. Renal cell apoptosis was detected by TUNEL method. The expression of caspase 3 mRNA in renal tissues was detected by RT PCR and the activity of caspase 3 was measured. Results Compared with CsA group, the combination of CsA and TP significantly improved CsA-induced renal injury and histological changes. The number of tubular and interstitial cells in CsA and TP group was significantly less than that in CsA group (7 7 ± 14vs 1 8 9 ± 3 3, P <0 05), and significantly decreased the expression of CsA-induced caspase 3 mRNA and its activity (P 0 05). Conclusion Antioxidant tea polyphenols can inhibit renal tubular and interstitial cell apoptosis when CsA induces chronic nephrotoxicity, suggesting that reducing the apoptosis induced by CsA is one of the mechanisms by which tea polyphenols can protect renal function and tissue structure.