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黄曲霉侵染花生的研究表明,种皮破损的黄曲霉毒素含量显著高于种皮完整的,种皮对黄曲霉侵染和产毒起着重要的屏障作用。采用氯仿去除种皮蜡质,用KOH或角质酶去除种皮角质层后,种子黄曲霉感染率和黄曲霉毒素含量显著提高。种皮蜡质和角质层同时去除的与种皮破损的黄曲霉感染率和毒素含量差异不显著,表明种皮的抗性成份主要是蜡质和角质层。种皮蜡质含量测定和种皮表面扫描电镜观察表明,蜡质的含量和角质层的厚度与品种的抗性有关。抗性品种种皮蜡质含量显著高于感病品种。种皮蜡质提取物在体外抑菌效果不显著,说明蜡质的抗性作用主要是物理性阻止黄曲霉菌的穿透。
Aspergillus flavus infection peanut research shows that the seed coat damaged aflatoxin content was significantly higher than the whole seed coat, the seed coat on Aspergillus flavus infection and toxin production plays an important barrier role. Chloroform removal of seed coat wax, KOH or cutinase removal cuticle stratum corneum, the seed of Aspergillus flavus infection rate and aflatoxin content increased significantly. There was no significant difference in the infection rate and the toxin content of the aspergillus flavus which were simultaneously removed from the seed coat wax and the stratum corneum and from the seed coat, indicating that the seed coat resistance mainly consisted of waxy and stratum corneum. The determination of the seed coat wax content and the observation of the surface of the seed coat by scanning electron microscopy showed that the wax content and the thickness of the stratum corneum were related to the variety resistance. The wax content of resistant cultivars was significantly higher than that of susceptible cultivars. Seed coat wax extract in vitro antibacterial effect is not significant, indicating that the main role of waxy resistance is to physically prevent the penetration of Aspergillus flavus.