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目的探讨载脂蛋白E(apolipoproteinE,apoE)基因多态性与新疆维吾尔族自然长寿的关系。方法应用聚合酶链反应限制性片段长度多态性方法检测百岁组42名,90岁组102名,65～70岁组70名和对照组53名的apoE基因多态性。结果百岁组apoE的ε3/3、ε2/3和ε3/4基因型频率分别为69.0%、23.8%和2.4%,其ε3、ε2和ε4等位基因频率分别为82.1%、16.7%和1.2%,百岁组ε3/4基因型及ε4、ε3等位基因频率显著低于对照组(P<0.01),ε2/3基因型及ε2等位基因频率则显著高于对照组(P<0.01)。百岁与apoE基因的ε2等位基因呈正关联,与ε4等位基因呈负关联。结论在新疆维吾尔族,apoE基因多态性与个体寿命密切相关,同时也应考虑到长寿是年龄依赖的多种因素影响的结果。
Objective To investigate the relationship between apolipoprotein E (apoE) gene polymorphism and natural longevity of Uighur in Xinjiang. Methods The polymorphism of apoE gene in 42 centenarians, 102 in 90-year-old group, 70 in 65- 70-year-old group and 53 in control group were detected by polymerase chain reaction restriction fragment length polymorphism. Results The frequencies of ε3 / 3, ε2 / 3 and ε3 / 4 genotypes in a hundred centenarians were 69.0%, 23.8% and 2.4%, respectively. The frequencies of alleles ε3, ε2 and ε4 were 82.1%, 16.7% and 1.2% %, The frequencies of ε3 / 4 genotypes and ε4, ε3 alleles in centenarians were significantly lower than those in control group (P <0.01), and those in ε2 / 3 genotypes and ε2 alleles were significantly higher than those in control group ). One-hundred-year-old was positively correlated with ε2 allele of apoE gene and negatively correlated with ε4 allele. Conclusion In Uygur Xinjiang, apoE gene polymorphism is closely related to individual life expectancy, and should also take into account that longevity is the result of many age-dependent factors.