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许多研究表明,大鼠肺对乙醇的代谢主要通过非氧化途径即通过脂肪酸乙酯(FAEE)的形成及硫酸结合作用,而氧化途径即通过乙醇脱氢酶(ADH)及微粒体代谢作用占很小比例。本文试图通过体外实验测定兔肺中脂肪酸乙酯合成酶及 ADH 的活性,评价兔肺对乙醇的氧化和非氧化代谢作用。本研究选用体重2.5～3.5 kg 的雄性新西兰家兔。首先,在不同的缓冲体系和 pH 值、底物乙醇的两种浓度即5 mmol/L 和500 mmol/L 的条件下,测定了兔肝和肺组织匀浆中的ADH 活性。结果表明,在生理 pH 值范围(7.2～7.4),肺内 ADH 活性很低并显著低于肝ADH 活性(P<0.05)。在较高的 pH 值范围(9.0～10.0),肺 ADH 活性升高,但仍低于肝 ADH 活性。由此可见,兔肺对乙醇的氧化代谢能力很低。其次,本文采用色层-放射自显影法,通过测定 FAEE 的生成量反映脏器中的脂肪酸乙酯合成酶的活性。实验观察了乙
Numerous studies have shown that rat lung metabolism of ethanol occurs primarily through the non-oxidative pathway through the formation of fatty acid ethyl esters (FAEEs) and the sulfate-binding effect, and the oxidative pathway, through the action of alcohol dehydrogenase (ADH) and microsomal metabolism Small proportion. In this paper, we attempted to measure the activity of fatty acid ethyl ester synthase (ADH) and ADH in rabbit lung by in vitro experiments and evaluate the oxidative and non-oxidative metabolism of ethanol in rabbit lung. In this study, male New Zealand rabbits weighing 2.5-3.5 kg were used. First, ADH activity in rabbit liver and lung homogenates was measured at two concentrations of 5 mmol / L and 500 mmol / L at different concentrations of buffer and pH, and substrate ethanol. The results showed that in the physiological pH range (7.2 ~ 7.4), ADH activity in lungs was low and significantly lower than that of liver ADH (P <0.05). Pulmonary ADH activity increased at higher pH values (9.0-10.0) but remained lower than hepatic ADH activity. Thus, rabbit lung ethanol oxidation and metabolism of low ability. Secondly, the color-layer autoradiography method was used to determine the activity of fatty acid ethyl ester synthase in viscera by measuring the amount of FAEE produced. Experimental observation of B