慢生型花生根瘤菌16S rRNA分析

• 作 者: 陈强 张小平 李登煜 Ｔｅｒｅｆｅｗｏｒｋ Ｚ Ｌｉｎｄｓｔｒｍ Ｋ
• 机 构: 四川农业大学农学院,芬兰赫尔辛基大学
• 出 处: 应用与环境生物学报
• 发表日期: 1999年03期
• 关键词: 根瘤菌属 大豆根瘤菌 遗传相似性 碱基序列 云南元谋 细胞基因组 酶切图谱 四川攀枝花 对数生长期 基因序列分析

以２１株分离自四川、云南不同花生产区的慢生型花生根瘤菌和６株参比菌株为材料，进行了１６ＳｒＤＮＡＰＣＲＲＦＬＰ，结果除证实了慢生型花生根瘤菌同慢生型大豆根瘤菌（Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｊａｐｏｎｉｃｕｍ）高度的遗传相似性外，还发现一些菌株同Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｌｉａｏｎｉｎｇｅｎｓｉｓ和Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｅｌｋａｎｉ有较高的遗传相似性，菌株Ｓｐｒ１２的１６ＳｒＲＮＡ部分碱基序列同Ｂｒａｄｙｒｈｉｚｏｂｉｕｍｅｌｋａｎｉ模式菌株ＵＳＤＡ７６的部分序列完全一样，从而证明我国的花生根瘤菌之间具有种水平的多样性．

Twenty-one strains of slow-growing peanut rhizobia and six reference strains isolated from different flower-producing areas in Sichuan and Yunnan Provinces were used as materials for 16S rDNA PCR-RFLP. The results showed that, in addition to slow-growth peanut rhizobia and slow-growing soybean nodules (Bradyrhizobiumjaponicum), it was also found that some strains had high genetic similarity with Bradyrhizobiumliaoningensis and Bradyrhizobiumelkani. The partial sequence of 16S rRNA gene of strain Spr1-2 was exactly the same as the partial sequence of USDA76 strain of Bradyrhizobiumelkani, Rhizobium species in our country have the kind of level of diversity.