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目的 :探讨慢性HBV感染者DC的表现和功能及其与免疫耐受的关系。方法 :选择正常人和慢性HBV感染者 ,从外周血中分离单个核细胞 ,用细胞贴壁法富集DC ,流式细胞术测定DCHLAⅡ和共刺激分子的表达 ,同位素掺入法测定DC的抗原提呈能力 ,ELISA方法测定抗原提呈过程中Th1和Th2类细胞因子的水平。结果 :慢性HBV感染者HLAⅡ和共刺激分子表达水平、抗原提呈能力和Th1类细胞因子的水平均低于正常 (P <0 .0 5 ) ,而Th2类细胞因子的水平与正常相似 (P >0 .0 5 )。结论 :慢性HBV感染免疫耐受与DC的缺陷有关。
Objective: To investigate the expression and function of DC in chronic HBV infection and its relationship with immune tolerance. Methods: Normal and chronic HBV infected individuals were selected, mononuclear cells were isolated from peripheral blood, DCs were enriched by cell adhesion method, the expression of DCHLA Ⅱ and costimulatory molecules were determined by flow cytometry, and the antigen of DC Presentation ability, ELISA method to determine the level of Th1 and Th2 cytokines during antigen presentation. Results: The levels of HLA Ⅱ and costimulatory molecules, the antigen presenting ability and the levels of Th1 cytokines in patients with chronic HBV infection were lower than those in normal controls (P <0.05), while the levels of Th2 cytokines in patients with chronic HBV infection were similar (P > 0 .0 5). Conclusion: The immune tolerance of chronic HBV infection is related to the defects of DC.