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目的：探讨乙肝标志物不同血清模式与HBV-DNA结果者之间的关系及临床意义。方法收集2014年6月~8月乙肝患者标本251例,采用时间分辨免疫方法(TRFIA)和实时荧光定量PCR方法(FQ-PCR)分别检测乙肝病毒血清标记物和病毒DNA,比较它们之间的关系。结果 HBsAg、HBeAg、HBcAb阳性组79例,HBV DNA定量阳性共72例；HBsAg、HBeAb、HBcAb阳性组101例,HBV DNA定量阳性63例,HBV DNA定量阴性38例；HBcAb阳性,其余全阴组35例,HBV DNA定量阳性19例,HBV DNA定量阴性14例；五项全阴组36例, HBV DNA定量阴性36例。HBV DNA在HBcAb阳性组与HBcAb阴性组间相比,有显著的统计学差异(﹤0.05)。FQ-PCR法和TRFIA法诊断乙肝的敏感性分别为71.6%和100%,符合率为85.8%。结论 FQ-PCR法和TRFIA法在HBcAg阳性组有良好的相关性,两种方法分别检测乙肝病毒感染机体不同状态,它们之间能互为补充,不能相互替代。“,”Objective To investigate the relationship and clinical significance of serum HBV markers in dif erent pat erns and HBV-DNA results among them.Methods From June 2014 to August 2014 specimens of 251 cases of hepatitis B patients,the use of time-resolved immunoassay (TRFIA)and real-time quantitative PCR (FQ-PCR)were used to detect hepatitis B virus serum markers and viral DNA,compare them Relationship between.Results HBsAg,HBeAg,HBcAb 79 cases positive,HBV DNA quantification positive total 72 cases;HBsAg,HBeAb,101 HBcAb positive patients,HBV DNA quantification positive 63 cases,HBV DNA quantification negative 38 cases;HBcAb positive,the rest overcast group of 35 patients,HBV DNA quantification positive 19 cases,HBV DNA quantification negative 14 cases;five ful female group of 36 patients,HBV DNA quantification negative 36 cases.HBV DNA in HBcAb between positive and negative HBcAb compared with a statistical y significant dif erence ( ﹤0.05).FQ-PCR method and sensitivity TRFIA diagnosis of hepatitis B were 71.6%and 100%,the rate was 85.8%.Conclusion FQ-PCR method and TRFIA method HBcAg positive group has a good cor elation between the two methods were used to detect hepatitis B virus infection in dif erent states of the body,can complement each other between them,can not replace each other.