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本研究对13例人的正常涎腺经三种固定双固定后的组织标本进行了11种角蛋白单克隆抗体的免疫组化染色.其中5例腮腺和3例颌下腺分别同时用常规Formalin固定,Bouin’s液固定,Carnoy‘s液固定.染色采用ABC法,结果发现:Carnoy固定者,K_8、K_18、K_19和K_8.12的染色效果最好.其导管染色比Bouin‘s和Formalin固定者染色强而明确,尤其K_8和K_8.12不仅在导管中呈现强阳性,而且腺泡也呈阳性;而K_(17)和K_(L1)没有看出明显的染色差别;K_(14)、K_(10)、K_(13)、K_(17)、K_(20)的染色几乎全为阴性,与常规中性缓冲Formalin固定者结果一致.
In this study, 11 kinds of keratin monoclonal antibodies were immunohistochemically stained with 11 kinds of keratin monoclonal antibodies in 13 salivary glands of normal salivary glands by fixed three pairs of fixation.Among them 5 parotid glands and 3 cases of submandibular glands were fixed with regular Formalin, Bouin's solution was fixed with Carnoy's solution, and the ABC method was used for staining. The results showed that Carnoy's fixer, K_8, K_18, K_19 and K_8.12 had the best staining effect, and its duct dyeing was better than that of Bouin's and Formalin fixer However, K_8 and K_8.12 were not only strongly positive in the duct, but also positive in the acinar. K_ (17) and K_ (L1) showed no significant difference in staining. K_ (14), K_ (10) ), K_ (13), K_ (17) and K_ (20) were all negative, which was consistent with the results of routine neutral buffered Formalin fixator.