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目的初步评价载双黄连粉针剂骨水泥珠链局部治疗慢性骨髓炎兔模型的有效性。方法将58只健康纯种新西兰大白兔根据骨水泥缓释系统载药不同随机分为正常组、模型组、万古霉素组、低浓度双黄连粉针剂组、高浓度双黄连粉针剂组、低浓度双黄连粉针剂与万古霉素联合组、高浓度双黄连粉针剂与万古霉素联合组,先造模再分组治疗。分别在治疗前,治疗后第2周、第4周、第8周心脏采血,观测兔血清C-反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)与白细胞介素-6(IL-6)的含量变化。结果在治疗全程,与正常组比较,模型组兔血清CRP,TNF-α,IL-6含量显著升高,差异均有高度统计意义(P<0.01);与模型组比较,各治疗组兔血清CRP,TNF-α含量显著降低,差异均有统计意义(P<0.05),治疗后第8周时,各治疗组兔血清CRP,TNF-α含量均明显降低,其中以低浓度双黄连粉针剂与万古霉素联合组、高浓度双黄连粉针剂与万古霉素联合组降低更为明显(P<0.05)。结论载高浓度双黄连与万古霉素制作的缓释系统局部治疗慢性骨髓炎兔模型效果优于载万古霉素缓释系统。
OBJECTIVE: To evaluate the effectiveness of Shuang Huang Lian injection in the treatment of chronic osteomyelitis in rabbits. Methods Fifty-eight healthy pure New Zealand white rabbits were randomly divided into normal group, model group, vancomycin group, Shuanghuanglian injection group and Shuanghuanglian injection group Shuanghuanglian powder injection combined with vancomycin, Shuanghuanglian high-dose powder injection and vancomycin combined group, the first model and then sub-group treatment. Blood samples were taken from the heart before treatment, 2 weeks, 4 weeks and 8 weeks after the treatment. The serum levels of C-reactive protein (CRP), tumor necrosis factor-α (TNF-α) and interleukin- -6) content changes. Results Compared with the normal group, the levels of CRP, TNF-α and IL-6 in the model group were significantly increased (all P <0.01). Compared with the model group, (P <0.05). At the 8th week after treatment, the levels of CRP and TNF-α in the serum of rabbits in each treatment group were significantly decreased, and the levels of CRP and TNF-α were significantly decreased Compared with vancomycin group, the combination of high concentration Shuanghuanglian injection and vancomycin group was more obvious (P <0.05). CONCLUSION: The rabbit model with chronic osteomyelitis treated with sustained release system made by Shuanghuanglian and vancomycin is better than vancomycin-containing sustained-release system.