矽肺与NO、氧化及脂质过氧化关系的探讨

• 作 者: 周君富 吴丹参 彭芬英 丁德云
• 机 构: 浙江医科大学附属第二医院,浙江省杭州市职业病防治院
• 出 处: 中华劳动卫生职业病杂志
• 发表日期: 1999年01期
• 关键词: 矽肺患者 NO 脂质过氧化 过氧化氢酶 矽尘 黄嘌呤氧化酶 一氧化氮合成酶 职业病防治院 抗氧化平衡 直线回归

目的探讨矽肺与ＮＯ及氧化和脂质过氧化的关系。方法检测７３例矽肺患者和６０例健康对照者的血浆ＮＯ、ＶｉｔＣ、ＶｉｔＥ、β胡萝卜素（βＣＡＲ）、过氧化脂质（ＬＰＯ）含量及红细胞超氧化物歧化酶（ＳＯＤ）、过氧化氢酶（ＣＡＴ）、谷胱甘肽过氧化物酶（ＧＳＨＰｘ）活力和ＬＰＯ含量。结果与对照组比较，矽肺组的血浆ＶｉｔＣ、ＶｉｔＥ、βＣＡＲ含量及红细胞ＳＯＤ、ＣＡＴ、ＧＳＨＰｘ活力显著降低，血浆ＮＯ、ＬＰＯ含量及红细胞ＬＰＯ含量显著升高（Ｐ＜０．０５或Ｐ＜０．０１）；各检测值与病程均有相关；血浆ＮＯ、ＶｉｔＥ含量及红细胞ＳＯＤ活力、ＬＰＯ含量与病程相关最密切。结论矽肺患者体内的ＮＯ代谢异常，氧化和脂质过氧化反应病理性加剧。

Objective To explore the relationship between silicosis and NO oxidation and lipid peroxidation. Methods Serum levels of NO, VitC, VitE, β-carotene (βCAR), lipid peroxidation (LPO) and erythrocyte superoxide dismutase (SOD) were measured in 73 patients with silicosis and 60 healthy controls. Catalase (CAT), glutathione peroxidase (GSH-Px) activity and LPO content. Results Compared with the control group, the levels of VitC, VitE and βCAR in plasma and the activities of SOD, CAT and GSHPx in erythrocytes were significantly decreased, and the content of NO and LPO in plasma and the content of LPO in erythrocytes were significantly increased in silicosis group (P <0.05 or P <0.01). The measured values ​​correlated with the course of the disease. The contents of NO and VitE in plasma and SOD activity in erythrocytes and LPO content were most closely related to the course of disease. Conclusions In patients with silicosis, NO metabolism is abnormal and the pathological changes of oxidation and lipid peroxidation are aggravated.