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利用16对SSR和10对SCoT引物对99个菊花品种的基因组变异进行扫描,分析其群体结构,并采用一般线性模型GLM和混合线性模型MLM方法对10个表型性状进行关联分析。供试群体Shannon’s指数为1.46~2.52。群体遗传结构分析将99个菊品种划分为5个亚群。GLM分析发现有8个SSR位点与7个表型性状相关联(P<0.01),其中与3个花部性状(花序直径、花序高度、舌花宽度)相关位点5个,与4个营养性状(叶柄长度、叶片长度、托叶大小及叶一级刻度)相关位点4个,各位点对表型变异的解释率在0.06~0.40之间;3个SCoT位点与花序直径相关联。MLM分析发现3个SSR位点与4个表型性状相关联。MLM中检测到的标记JH42、JH81和JH86在GLM中同样被检测到,但解释率稍低。
The genomes of 99 chrysanthemum varieties were scanned using 16 pairs of SSRs and 10 pairs of SCoT primers. The population structure was analyzed. Ten phenotypic traits were analyzed using general linear model (GLM) and mixed linear model (MLM). The Shannon’s index of the test population was 1.46 ~ 2.52. Population genetic structure analysis 99 chrysanthemums were divided into 5 subgroups. GLM analysis found that there were 8 SSR loci associated with 7 phenotypic traits (P <0.01), of which 5 were related to 3 flower traits (inflorescence diameter, inflorescence height, width of tongue flower) and 4 There were 4 loci related to nutritional traits (petiole length, leaf length, stipule size and leaf first scale), and the interpretation rates of phenotypic variation at each loci ranged from 0.06 to 0.40. Three SCoT loci were correlated with inflorescence diameter . MLM analysis found that 3 SSR loci were associated with 4 phenotypic traits. The markers JH42, JH81 and JH86 detected in MLM were also detected in GLM, but with a slightly lower interpretation rate.