Isolation and identification of antibacterial components in seed extracts of Argemone mexicana L.(Pa

• 作 者: Indranil Bhattacharjee Soroj Kumar Chatterjee Goutam Chandra
• 机 构: Mosquito & Microbiology Research Units,Parasitology Laboratory,Department of Zoology,The University
• 出 处: Asian Pacific Journal of Tropical Medicine
• 发表日期: 2010年07期
• 关键词: extracts antibacterial screening potent alkaloid retention chloroform Klebsiella

Objective:The column chromatographic fractions of chloroform(CH1,CH2,CH3) seed extracts of Argemone mexicana(A.mexicana) were screened for antibacterial activity and phytochemical analysis.Methods:CH3 fraction was isolated and identified by column chromatography,thin layer chromatography,spectral data analysis and phytochemical screening were used for analysis. Results:CH3 fraction was significantly active at 4 to 64 mg/mL against Staphylococcus aureus, Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa with minimum inhibitory concentration of 1.5625 to 3.125 mg/mL.The active fraction(CH3) revealed presence of alkaloid with retention factor value of 0.44.The active antibacterial agent in the most potent fraction (CH3) was isolated and identified as N-demethyloxysanguinarine by thin layer chromatography (TLC) and phytochemical screening.The CH1 and CH2 fractions did not show inhibitory activity. Conclusions:The results support the ethnomedicinal use of seeds of A.mexicana for treatment of bacterial diseases.

Objective: The column chromatographic fractions of chloroform (CH1, CH2, CH3) seed extracts of Argemone mexicana (A. mexicana) were screened for antibacterial activity and phytochemical analysis. Methods: CH3 fraction was isolated and identified by column chromatography, thin layer chromatography, Spectral data analysis and phytochemical screening were used for analysis. Results: CH3 fraction was significantly active at 4 to 64 mg / mL against Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa with a minimum inhibitory concentration of 1.5625 to 3.125 mg / mL. Active fraction (CH3) revealed presence of alkaloid with retention factor value of 0.44. The active antibacterial agent in the most potent fraction (CH3) was isolated and identified as N-demethyloxysanguinarine by thin layer chromatography (TLC) and phytochemical screening.The CH1 and CH2 fractions did not show inhibitory activity. Conclusions: The results support the ethnomedicinal use of seeds of A. mexicana for treatment of bacterial diseases.