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目的:探讨3,4′,5-三羟基芪-3-β-单-D-葡萄糖苷(polydatin,Pol)抑制血小板聚集与其使血管内皮释放前列环素作用间的关系.方法:Pol与培养的人脐静脉内皮细胞(VEC,酶消化法)孵育为内皮组,无VEC为对照组,移细胞培养液至兔血小板(洗涤法),测凝血酶诱聚后血小板聚集率(PAR,比浊法)及上清液6-酮-前列环素F_(1alpha)(6-keto-PGF_(1α)),血栓烷B_2(TXB_2)含量(放免法).结果:无VEC组Pol不减少PAR;VEC组0.41 mmol·L~(-1)10 minPAR减少(-10±10),6-keto-PGF_(1α)增加(108±30 ng·L~(-1))(各与蒸馏水组之2±12,54±20ng·L~(-1)比).结论:Pol抑制血小板聚集与其增加VEC前列环素释放有关.
OBJECTIVE: To investigate the relationship between inhibition of platelet aggregation by 3,4,5-trihydroxy-stilbene-3-β-D-glucoside (Pol) and its role in the release of prostacyclin from the vascular endothelium.Methods: Pol and culture Of human umbilical vein endothelial cells (VEC, enzymatic digestion) were incubated with endothelium, without VEC as a control group, the cell culture broth was transferred to a platelet of rabbits (washing method), the platelet aggregation rate after thrombin induction (PAR, (1α) and thromboxane B_2 (TXB_2) in the supernatant were determined by radioimmunoassay.Results: Pol did not reduce PAR in the non-VEC group, In VEC group, the decrease of PARPAR at 0.41 mmol·L -1 (-10 ± 10) and the increase of 6-keto-PGF 1α (108 ± 30 ng · L -1) ± 12,54 ± 20ng · L -1) .Conclusion: Pol inhibits platelet aggregation and increases the release of prostacyclin from VEC.