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本文报道将小麦白粉菌的分生孢子接种于生长在含50ppm苯骈咪唑的1.5％琼脂培养基试管幼苗中,于1-5℃不加光照的冰箱中保存。种子表面消毒处理与培养基灭菌与否,对保存时间的长短有显著区别:种子表面不消毒、培养基不灭菌者,一般保存135-150天;种子表面消毒、培养基灭菌者,保存时间达150-170天,最高达180天。提高保存温度,保存时间缩短。增加培养基中苯骈咪唑浓度,保存时间略有增加。在培养基中另加营养液,保存时间无变化。
In this paper, the conidia of wheat powdery mildew were inoculated on 1.5% agar medium containing 50 ppm benzimidazole and stored in the refrigerator without illumination at 1-5 ℃. Seed surface disinfection and sterilization medium or not, the length of the preservation time are significantly different: the surface of the seed is not sterile, the medium is not sterile, generally stored 135-150 days; seed surface disinfection, medium sterilization, Save time up to 150-170 days, up to 180 days. Increase storage temperature, saving time shortened. Increase the concentration of benzodiazepine in the medium, save a slight increase in time. In the medium plus nutrient solution, save time without change.