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探讨人参皂甙（ＧＳ）对人骨髓ＣＤ３４＋造血干细胞的增殖作用。方法：采给Ｄｙｎａｌ Ｍ４５０ ＣＤ３４＋免疫磁珠分离法阳性选择获取高纯度的人骨髓ＣＳ３４＋造血干细胞，应用多向祖细胞（ＣＦＵ－Ｍｉｘ）体外培养技术，观察ＣＤ３４＋造血干细胞对ＧＳ的敏感性。结果：１．经Ｄｙｎａｌ Ｍ－４５０ ＣＤ３４＋免疫磁珠分离获得骨３髓 ＣＤ３４＋细胞占骨髓单个核细胞（ ＭＮＣ）的 ０． ５２～１．３０％；ＣＤ３４＋细胞的存活率达９４．０～９８．２％。 ２最佳浓度的ＧＳ使ＣＤ３４＋细胞的ＣＦＵ－Ｍｉｘ集落形成率提高５４．４％（Ｐ＜０．０１），而高浓度时表现为抑制。结论：ＧＳ不但作用于人骨髓造血祖细胞，而已对ＣＤ３４＋造血干细胞也具有明显的刺激增殖作用。
To investigate the effect of ginsenosides (GS) on the proliferation of human bone marrow CD34 + hematopoietic stem cells. METHODS: Human bone marrow CS34 + hematopoietic stem cells with high purity were positively selected by Dynal M450 CD34+ immunomagnetic bead separation method. The sensitivity of CD34 + hematopoietic stem cells to GS was observed by in vitro culture of multi-progenitor cells (CFU-Mix). Results: 1. Bone marrow 3 marrow CD34+ cells were isolated from bone marrow mononuclear cells (MNC) by Dynal M-450 CD34+ immunomagnetic beads. 52 to 1.30%; the survival rate of CD34+ cells reached 94.0 to 98.2%. 2The optimal concentration of GS increased the CFU-Mix colony formation rate of CD34+ cells by 54.4% (P<0.01), but showed inhibition at high concentrations. CONCLUSION: GS not only acts on human bone marrow hematopoietic progenitor cells, but also has significant stimulation on CD34+ hematopoietic stem cells.