论文部分内容阅读
目的：采用高效液相色谱(HPLC)测定海尔福口服液中甘草酸含量,并与甘草药材提取液进行对比分析。方法采用高效液相色谱法,色谱柱：Acclaim?120 C185μm (4.6×250mm)流动相：甲醇-水-冰乙酸(71：28：1)波长：250nm 流速：1.0ml/min。结果（1）甘草药材1醇提液甘草酸含量为28.900±0.221 mg/g生药；（2）甘草药材1水提液甘草酸为19.360±0.029mg/g生药；（3）旧甘草药材水提液甘草酸含量为13.217±0.064mg/g生药；（4）海尔福口服液中甘草酸含量为11.887±0.186mg/g生药；（5）甘草药材2水提液甘草酸为25.907±0.619mg/g生药；各样品甘草酸含量比较,p<0.01,差异均有统计学意义；加样回收试验平均为109.88%,进样20μl测定浓度在0.04-0.32m g/m l按峰高值计有良好线性关系,相关系数为R=0.9984,精密度RSD%=0.45%。结论该法测定准确,线性关系好,可用于海尔福口服液质量控制.“,”Objective Determination of Glycyrrhizic acid content in Hai er fu oral liquid by HPLC, and comparative analysis with Licorice extract. Method Using high-performance liquid chromatography, the determination was performed on an Acclaim? 120 C18 5 μm (4.6×250mm) column, with the mobile phase was methanol - water -glacial acetic acid (71:28:1) at a flow rate of 1.0ml/min, and the detection wavelength was 250 nm. Result (1) The Glycyrrhizic acid content of Licorice 1 alcohol extract was 28.900±0.221 mg/g crude drug;(2) The water extract of licorice 1 Glycyrrhizic acid was 19.360±0.029mg/g crude drug ;(3) The Glycyrrhizic acid content of old licorice extract was 13.217±0.064mg/g crude drug;(4) The content of Glycyrrhizic acid in Hai er fu oral liquid was 11.887± 0.186mg/g crude drug;(5) The water extract of licorice 2 Glycyrrhizic acid was 25.907±0.619mg/g crude drug; compared the content of Glycyrrhizic acid in al samples, p<0.01, the differences were statisticaly significant; The results of recovery test for an average of 109.88%, Determination of the concentration of 20 μ l at 0.04- 0.32mg/ml according to the peak value meter had a good linear relationship between the sample, The correlation coefficient was R=0.9984, The precision of RSD% was 0.45%. Conclusion: This method has accurate determination and good linear relationship, can be used for the quality control of Hai er fu oral liquid.