Transcriptome-Wide Identification and Validation of Reference Genes in Black Rockfish(Sebastes schle

• 作 者: JIN Chaofan SONG Weihao WANG Mengya QI Jie ZHANG Quanqi HE Yan
• 机 构: MOEKeyLaboratoryofMarineGeneticsandBreeding,LaboratoryforMarineFisheriesScienceandFoodProductionProc
• 出 处: 中国海洋大学学报:英文版
• 发表日期: 2021年3期
• 关键词: reference genes black rockfish transcriptome-wide CV value QRT-PCR
• 基金项目: This study was supported by the National Key R&D Program of China(No.2018YFD0900101).

The quantitative real-time reverse transcription PCR(qRT-PCR)is a widely used technique to analyze gene expression levels.Selecting a suitable reference gene is a crucial step to obtain an accurate result in qRT-PCR.However,most previous studies on fishes