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为了解血源乙型肝炎（乙肝）疫苗的免疫持久性，对澄江县1991～1995年按照0、1、6个月免疫程序完成血源乙肝疫苗接种的人群，按接种后间隔1～5年分组，每组随机抽取100名儿童，采末梢血，共得517份免疫者的血清样品．用酶联免疫吸附试验（ELISA）检测抗-HBs、HBsAg。结果表明，接种乙肝疫苗2年内抗-HBs阳性率在90％以上，以后随间隔年眼的延长而明显下降，间隔5年降至60％，人群平均保护率达78.57％．
In order to understand the immune persistence of Hepatitis B (Hepatitis B) vaccine, a total of 1 to 5 years after completion of Hepatitis B vaccine vaccination in Chengjiang County from 1991 to 1995 according to the immunization schedule of 0, 1 and 6 months Divided into groups, each group randomly selected 100 children, collecting peripheral blood, a total of 517 immunized persons serum samples. Anti-HBs and HBsAg were detected by enzyme-linked immunosorbent assay (ELISA). The results showed that the positive rate of anti-HBs was over 90% in two years after inoculation of hepatitis B vaccine and then decreased significantly with the prolongation of interval eyes. After 5 years interval, the anti-HBs positive rate decreased to 60% and the average protection rate reached 78.57%.