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目的阐明异氟醚对不同脑区突触体Ｎａ＋，Ｋ＋－ＡＴＰ酶活性的影响。方法选用５０只雄性ＳＤ大鼠随机分为５组：对照组、１．０ＭＡＣ异氟醚组、１．５ＭＡＣ异氟醚组、２．０ＭＡＣ异氟醚组、２．０ＭＡＣ恢复组。分别测定皮层、海马、脑干、小脑、突触体Ｎａ＋，Ｋ＋－ＡＴＰ酶的活性。结果１．５ＭＡＣ、２．０ＭＡＣ异氟醚以剂量依赖的方式显著抑制上述４个脑区Ｎａ＋，Ｋ＋－ＡＴＰ酶的活性（Ｐ＜０．０１）。结论异氟醚可能通过对皮层、海马、小脑、脑干突触体Ｎａ＋，Ｋ＋－ＡＴＰ酶的抑制，影响突触兴奋、突触传导和突触囊泡释放神经递质而发挥麻醉作用。
Objective To elucidate the effects of isoflurane on the activity of Na +, K + -ATPase in synapse of different brain regions. Methods Fifty male Sprague-Dawley rats were randomly divided into five groups: control group, 1.0MAC isoflurane group, 1.5MAC isoflurane group, 2.0MAC isoflurane group and 2.0MAC recovery group. The activity of Na +, K + -ATPase in cortex, hippocampus, brainstem, cerebellum and synaptosome were measured respectively. Results 1.5MAC and 2.0MAC isoflurane significantly inhibited the activity of Na +, K + -ATPase in the above four brain regions (P <0.01) in a dose-dependent manner. Conclusion Isoflurane may exert anesthetic effects through inhibition of Na +, K + -ATPase in cortex, hippocampus, cerebellum and brainstem synapse, affecting synaptic excitability, synaptic transmission and synaptic vesicle release.