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[目的]探讨去甲斑蝥酸钠(SNCTD)对耐顺铂人肺腺癌细胞系A549/DDP的逆转作用及其对耐药细胞周期的影响。[方法]⑴采用CCK法筛选出SNCTD对A549/DDP的无毒浓度(即对细胞抑制率<10%的药物浓度),并检测出顺铂及与无毒浓度SNCTD联合对耐药细胞株的IC50。⑵光学显微镜下观察无毒浓度SNCTD组、单纯DDP处理组及联合用药组用药48h细胞形态的变化。⑶采用流式细胞仪观察用药48h后正常细胞组、无毒浓度SNCTD组、DDP组及DDP+SNCTD组对A549/DDP细胞周期的影响。[结果]⑴SNCTD对A549/DDP的无毒浓度为5μg/ml,与DDP联合用药降低A549/DDP的耐药逆转倍数为1.97。⑵无毒浓度SNCTD处理耐药细胞组光学显微镜下细胞形态未见明显改变,而联合用药组比单纯DDP作用后细胞形态明显变差。⑶无毒浓度SNCTD处理耐药细胞后细胞周期未见明显改变,单纯DDP处理后细胞阻滞在S期,联合用药后S期减少,G0/G1期细胞增多。[结论]SNCTD对A549/DDP具有耐药逆转作用,与DDP联用对耐药细胞有协同杀伤作用。
[Objective] To investigate the reversal effect of norcantharidin sodium (SNCTD) on cisplatin-resistant human lung adenocarcinoma cell line A549 / DDP and its effect on drug-resistant cell cycle. [Method] (1) The non-toxic concentration of SNCTD on A549 / DDP was screened by CCK method (that is, the drug concentration of inhibition rate of <10%), and the combination of cisplatin and SNCTD with drug- IC50. ⑵ under light microscope, SNCTD group, DDP group and combination group were observed 48h cell morphology changes. (3) Flow cytometry was used to observe the effects of SNCTD, DDP and DDP + SNCTD on the cell cycle of A549 / DDP cells in normal cells 48h after drug administration. [Results] (1) The nontoxic concentration of SNCTD to A549 / DDP was 5μg / ml, and the drug resistance reversal fold of A549 / DDP in combination with DDP was 1.97. (2) There was no significant change of cell morphology under light microscope in SNCTD-resistant cell-free group, but the cell morphology in the combined drug-treated group was significantly worse than that of DDP alone. (3) No significant changes in the cell cycle of drug-resistant cells treated with SNCTD at a non-toxic concentration. Cells were arrested in S phase after treatment with DDP alone, S phase decreased and G0 / G1 phase cells increased after DDP treatment. [Conclusion] SNCTD has a drug-resistant reversal effect on A549 / DDP cells and synergistic killing effect on drug-resistant cells with DDP.