PCR检测丙型肝炎病毒RNA与ELISA检测方法比较

• 作 者: 肖波 黄宏君
• 机 构: 湖南省肿瘤医院
• 出 处: 当代医师杂志
• 发表日期: 1998年11期
• 关键词: 丙型肝炎病毒 ELISA检测 PCR检测 感染滴度 急性期 正链 RNA 特异性 阴性标本 阴性结果

丙型肝炎病毒（ＨＣＶ）为一条单股正链线状ＲＮＡ由于ＨＣＶ在血液中感染滴度低，现有的ＥＬＩＳＡ方法在感染急性期抗体检出率仅能达到６０％，这就意味着部分病人可呈阴性结果。而今国内外已开展的ＰＣＲ方法其灵敏度高、特异性强，使这部分病人能得到早期、正确诊断的...

Hepatitis C virus (HCV) is a single positive stranded RNA Because of the low titers of HCV in the bloodstream, the current ELISA method detects only 60% of antibodies in the acute phase of infection, which means that part Patients can be negative results. Nowadays, PCR methods have been developed both at home and abroad with high sensitivity and specificity so that this part of patients can get early and correct diagnosis.