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目的:通过灵芝菌与玉米须、玉米芯的双向固体发酵,形成新的药性菌质,并对其活性成分进行分析。方法:以不同配比的玉米须和玉米芯为基质,以活性物质多糖和三萜的含量为指标考察菌质的最佳培养基配比,分别采用紫外分光光度法、薄层色谱法和高效液相色谱法进行定性及定量分析。结果:玉米须和玉米芯以1∶0,1∶1,1∶2,1∶3,1∶4,1∶5,0∶1配比,用灵芝菌发酵,形成的菌质多糖含量递增,三萜含量递减;薄层色谱结果显示,与发酵前对比,发酵后色谱斑点由红色转为蓝绿色荧光,并随着玉米须在培养基中比重增加而斑点越明亮;高效液相色谱结果显示,发酵后原有一些物质峰减弱或消失,并生成新的物质。结论:以玉米须-玉米芯1∶5作为基质产生的菌质长势及活性成分含量最高,发酵前后菌质的物质组成有明显改变。
OBJECTIVE: To establish a new drug-resistant bacterium by bi-directional solid fermentation of Ganoderma lucidum, corn cob and corncob, and to analyze its active ingredients. Methods: Corn stalks and corncobs with different ratios were used as substrate, and the optimum medium ratio of the mycoplasma was determined by the content of polysaccharide and triterpene in active substances. The optimum mediums were determined by UV spectrophotometry, TLC and high efficiency Liquid chromatography for qualitative and quantitative analysis. Results: Corn whisker and corn cob were in the ratio of 1: 0,1: 1,1: 2,1: 3,1: 4,1: 5,0: 1, , Triterpenoids decreased; TLC showed that compared with before fermentation, the chromatographic spots after fermentation changed from red to blue-green fluorescence, and with the proportion of corn in the medium to increase the more bright spots; HPLC results It shows that some of the original material peaks weaken or disappear after fermentation and generate new substances. CONCLUSION: The growth of the mycelium and the active ingredient content of maize - corncob 1: 5 as the substrate are the highest, and the material composition of the mycelium changes obviously before and after the fermentation.