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目的研究高压脉冲电场(high-voltage pulsed electric field,PEF)技术辅助提取玉米皮多糖。方法以玉米皮粉(纤维素含量21.25%)为原料,以多糖提取率为衡量指标,以电场强度、电场频率和液固比为3个重要因素,根据单因素及响应面试验设计构建PEF技术提高玉米皮粉酶解效果的回归模型,优化出最佳工艺参数;同时对提取的多糖进行中红外(mid-infrared spectroscopy,MIR)测定,与PEF辅助水提法比较分析提取多糖的结构差异。结果 PEF辅助水提法的最佳工艺参数为:电场强度25 k V/cm,电场频率2080 Hz,液固比42:1 mL/g,此条件下PEF辅助酶法对玉米皮多糖的提取率最高可达(15.36±0.25)%,与PEF辅助水提法相比提高了6.4%。中红外谱图结果表明,PEF辅助水提法和PEF辅助酶提法多糖在O-H振动及C=O振动上有着明显差别。结论PEF技术可以提高玉米皮多糖的提取率且PEF辅助酶提法效果更佳。
Objective To study the extraction of corn bran polysaccharides by high-voltage pulsed electric field (PEF). Methods Corn flour (cellulose content 21.25%) was used as raw material and polysaccharide extraction rate was taken as the index. Electric field strength, electric field frequency and liquid-solid ratio were three important factors. PEF was designed according to single factor and response surface test The regression model was established to optimize the enzymolysis effect of maize meal, and the optimum technical parameters were optimized. Meanwhile, the polysaccharide was determined by mid-infrared spectroscopy (MIR) and compared with the PEF-assisted water extraction. Results The optimal parameters of PEF assisted water extraction were electric field intensity of 25 k V / cm, electric field frequency of 2080 Hz and liquid-solid ratio of 42: 1 mL / g. Under these conditions, the extraction rate of polysaccharide from corn bran Up to (15.36 ± 0.25)%, an increase of 6.4% over the PEF-assisted water extraction. The results of mid-infrared spectroscopy showed that PEF-assisted water extraction and PEF-assisted enzymic polysaccharide showed obvious differences in O-H vibration and C = O vibration. Conclusion PEF technology can improve the extraction rate of corn bran polysaccharides and PEF-assisted enzyme extraction method is better.