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目的：对独特型抗体可变区基因进行序列分析，为其基因工程抗体的构建打下基础。方法：采用ＴＲＩｚｏｌＲ试剂从分泌抗独特型抗体ＳＭ６杂交瘤细胞株中提取ｍＲＮＡ，以特异性引物采用ＰＣＲ技术扩增并克隆出独特型单抗ＳＭ６重、轻链可变区（ＶＨ、ＶＬ）基因。将ＶＨ和ＶＬ基因分别克隆到ｐＧＥＭＴ载体中。采用链末端终止法荧光染色测定ＶＨ和ＶＬ基因序列，应用ＤＮＡＳＩＳ７分析软件并与美国国立卫生研究院基因库比较分析。结果：５个克隆的重链基因序列一致，３个克隆的轻链基因中有２个克隆基因一致。结论：ＶＨ和ＶＬ基因分别属Ｑ５２、ＪＨ３和ＶＫ１９、ＪＫ２。
OBJECTIVE: To sequence the variable region genes of idiotypic antibodies to lay the foundation for the construction of genetically engineered antibodies. METHODS: mRNA was extracted from secretory anti-idiotype antibody SM6 hybridoma cell line using TRIzolR reagent, and the specific heavy chain variable (VH, VL) gene of SM6 monoclonal antibody was amplified and cloned by PCR using specific primers. . The VH and VL genes were cloned into the pGEM-T vector, respectively. The VH and VL gene sequences were determined by fluorescent staining at the end of the chain. The DNASIS7 analysis software was used and compared with the National Institutes of Health Gene Bank. Results: The sequences of the heavy chain genes of the five clones were identical, and two cloned genes were identical in the light chain genes of the three clones. Conclusion: The VH and VL genes belong to Q52, JH3, VK19 and JK2, respectively.