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目的建立通林片的质量标准。方法用TLC法鉴别通林片中的黄芪、丹参、川芎和赤芍;用HPLC法测定赤芍中芍药苷的含量,选用Agilent Eclipse Plus C18柱(4.6 mm×250 mm,5μm);以甲醇-0.05 mol·L-1磷酸二氢钾溶液为流动相梯度洗脱;流速:1.0 ml·min-1;柱温:25℃;检测波长:230 nm。结果 4种药材的TLC图谱斑点清晰,阴性对照无干扰;芍药苷在12.89~206.30μg·ml-1范围内有良好的线性关系(r=0.9997)。结论该方法操作简单、灵敏准确,可以用于通林片的质量控制。
Objective To establish the quality standard of Tonglin. Methods The contents of paeoniflorin in Radix Paeoniae Alba were determined by TLC method on Radix Astragali, Radix Salviae Miltiorrhizae, Rhizoma Chuanxiong and Radix Paeoniae Rubra by HPLC. The column was on an Agilent Eclipse Plus C18 column (4.6 mm × 250 mm, 5 μm) 0.05 mol·L-1 potassium dihydrogen phosphate solution as mobile phase gradient elution; flow rate: 1.0 ml · min-1; column temperature: 25 ℃; detection wavelength: 230 nm. Results Four kinds of medicinal materials showed clear spots on the TLC chromatogram and no interference in the negative control. Paeoniflorin had a good linear relationship (r = 0.9997) in the range of 12.89 ~ 206.30μg · ml-1. Conclusion The method is simple, sensitive and accurate, and can be used for the quality control of Tonglin.