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目的：为测定人肝细胞微粒体细胞色素Ｐ４５０氧化酶的活性。方法：用差速离心法制备３例人肝细胞微粒体。结果：细胞色素Ｐ４５０的含量为０．５２３±０．００５ｎｍｏｌ·ｍｇ－１；细胞色素ｂ５为０．２８５±０．０２５ｎｍｏｌ·ｍｇ－１；氨基比林Ｎ－脱甲基酶的活力为０．５±０．６ｎｍｏｌ·ｍｇ－１；乙基吗啡Ｎ－脱甲基酶活力为０．９８±０．０８ｎｍｏｌ·ｍｇ－１。结论：Ｐ４５０酶活性影响因素较多，个体差异大。临床用药时应考虑患者的个体情况。
Objective: To determine the activity of microsomal cytochrome P450 oxidase in human hepatocytes. Methods: Three human hepatocyte microsomes were prepared by differential centrifugation. Results: The content of cytochrome P450 was 0.523 ± 0.005 nmol · mg-1 and the cytochrome b5 was 0.285 ± 0.025 nmol · mg-1. The activity of aminopyrine N-demethylase was 0. 5 ± 0.6nmol · mg-1; Ethylmorphine N-demethylase activity was 0.98 ± 0.08nmol · mg-1. Conclusion: There are many influencing factors of P450 enzyme activity, and individual differences are large. Clinical medication should take into account the individual patient’s condition.