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目的:建立稳定表达缺失型DNA聚合酶β(polβ)的细胞系。方法:将已构建的缺失型polβ的真核表达载体pcDNA3.1-polβ用脂质体法转染中国仓鼠卵巢细胞(CHO),经G418筛选后得到稳定转染的细胞株,用RT-PCR方法鉴定细胞缺失型polβmRNA的表达。结果与结论:成功转染和筛选出表达缺失型polβ的细胞株CHO-polβ,建立了稳定表达缺失型polβ的CHO细胞株。
OBJECTIVE: To establish a cell line stably expressing DNA polymerase β (polβ). Methods: The eukaryotic expression vector pcDNA3.1-polβ of the constructed deletion polβ was transfected into Chinese hamster ovary cells (CHO) by lipofectamine. The stable transfected cell lines were obtained by G418 selection and transfected into CHO cells by RT-PCR Methods The expression of cell-deficient polβmRNA was identified. RESULTS AND CONCLUSION: The CHO-polβ cell line expressing polβ was successfully transfected and selected. The CHO cell line stably expressing deletion polβ was established.