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目的探究一种有效分离纯化菊米黄酮的大孔树脂并对纯化工艺条件进行优化。方法以树脂的饱和吸附容量、吸附速率和解吸附率为参考指标,从10种不同的大孔吸附树脂中筛选出综合性能较佳的树脂,并进一步考察此树脂纯化菊米总黄酮的影响因素和分离纯化效果。结果 HPD-100树脂的综合性能较好,饱和吸附容量达到60.2 mg/g(以湿重计),且吸附迅速,解吸附完全。较理想的吸附工艺:在树脂径高比为1∶10的条件下,质量浓度为4.3 mg/mL供试液以15 mL/min上柱时,最佳上样量为5倍柱床体积(BV),静态吸附时间为2 h;较理想的洗脱工艺:先用6 BV的纯水洗杂质,再以60%的乙醇为洗脱剂,洗脱体积流量为8 mL/min时,用量为3 BV,所得菊米总黄酮质量分数为(81.6±0.9)%。结论 HPD-100是一种分离纯化菊米黄酮较佳的树脂,其工艺简单,具有较好的工业化生产前景。
OBJECTIVE To investigate an effective separation and purification of macroporous resins of chrysanthemum indicum and to optimize the purification process conditions. Methods Based on the saturated adsorption capacity, adsorption rate and desorption rate of resin, the resin with better overall performance was screened from 10 different macroporous adsorption resins. The factors affecting the purification of total flavonoids from the resin were further investigated. Separation and purification effect. Results HPD-100 resin showed better overall performance, with a saturated adsorption capacity of 60.2 mg / g (wet basis) and rapid adsorption with complete desorption. The ideal adsorption process: the resin diameter ratio of 1:10, the concentration of 4.3 mg / mL for the test solution to 15 mL / min on the column, the best sample volume of 5 times the bed volume ( BV), the static adsorption time was 2 h; the more ideal elution process: first wash the impurities with 6 BV pure water, and then with 60% ethanol as eluent, the elution volume flow rate was 8 mL / min, the dosage was 3 BV, and the obtained total flavonoids content was (81.6 ± 0.9)%. Conclusion HPD-100 is a better resin for the separation and purification of chrysanthemum indicum. Its technology is simple and has good industrial production prospects.