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用大白菜和荞麦 ,设 2 5、50、1 0 0、1 50、2 0 0株 5种不同群体 ;采用纱网隔离 ,人工链式、成对杂交及人工辅助或虫媒等不同授粉方式的田间种植试验。观察、记载主要农艺性状 ,测定各繁种方法与原种繁殖前后 9种同工酶 ,计算遗传多样性指数 ,比较不同种群间及原种与繁殖后代间的差异显著性 (遗传相似性 )。提出这两种作物基因库种子的最佳繁种措施 :荞麦在网罩内种 50～ 1 0 0株 ,采用家蝇传粉 ;大白菜种植 50～ 1 0 0株 ,群体内进行人工链式杂交
With Chinese cabbage and buckwheat, 5 different groups of 25, 50, 100, 150 and 200 strains were set up. Different pollination methods such as gauze isolation, artificial chain, paired crosses and artificial supplements or insect vectors Field planting test. The main agronomic traits were observed and recorded, and 9 isoenzymes were measured before and after multiplication and breeding. The index of genetic diversity was calculated and the significance (genetic similarity) between different populations and between breeding and breeding progenies was compared. The best breeding measures for the two crop genebanks were proposed: 50 ~ 100 strains of buckwheat were planted in the grille, pollinated by housefly, 50 ~ 100 strains of cabbage were planted, artificial linkage analysis