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目的建立测定人血浆中伏立康唑浓度的RP-HPLC法,并测定AIDS合并肺部真菌感染患者伏立康唑的血药浓度。方法留取患者稳态谷和稳态峰浓度时的血样,以岛津LC-20A高效液相色谱仪进行测定。色谱柱为Diamonsil C_(18)柱,流动相为0.01 mol·L~(-1)醋酸铵-乙腈(50:50,V/V),流速0.8 mL·min~(-1),紫外检测波长255 nm,内标为酮康唑,柱温40℃,进样量50μL。结果色谱峰分离良好,血浆内源性杂质无干扰,回归方程为y=8.400 2x+2.26×10~(-2)(r=0.999 9),血浆中伏立康唑在0.10～19.87 mg·L~(-1)内线性关系良好,日内和日间精密度(RSD)均<5%。结论本方法灵敏、准确、重现性好,适于伏立康唑血药浓度的测定。
Objective To establish a RP-HPLC method for the determination of voriconazole in human plasma and to determine the plasma concentration of voriconazole in AIDS patients with pulmonary fungal infection. Methods The blood samples from patients with steady-state valleys and steady-state peak concentrations were collected and measured by Shimadzu LC-20A high performance liquid chromatograph. The column was Diamonsil C_ (18) with a mobile phase of 0.01 mol·L -1 ammonium acetate-acetonitrile (50:50 V / V) at a flow rate of 0.8 mL · min -1. The UV detection wavelength 255 nm, internal standard ketoconazole, column temperature 40 ℃, injection volume 50μL. Results The chromatographic peaks were well separated and the plasma endogenous impurities were not disturbed. The regression equation was y = 8.400 × 2.26 × 10 -2 (r = 0.999 9), voriconazole in the plasma ranged from 0.10 to 19.87 mg · L -1 1) within a good linear relationship, intra-day and day precision (RSD) <5%. Conclusion The method is sensitive, accurate and reproducible. It is suitable for the determination of voriconazole blood concentration.