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在静态平衡条件下,应用FT-IR和减差光谱技术系统地测定了Poly A薄膜在温度为25±1℃、相对湿度从0％到100％的不同情况下的吸收光谱,波段范围为4000-800cm~(-1)。根据红外光谱的峰位和强度的变化,证实了Poly A的水化作用主要发生在碱基中的-NH_2基团和磷酸根基团上。其中-NH_2的光谱强度在相对湿度低于30％时就有明显变化,是水化较强的基团之一;而P=0的峰位移最大,并且在高湿度时出现两个振动频率,说明有两种不同的水化状态。在水化过程中,水峰的峰位也有较大变化,并分裂为多重峰。
Under static equilibrium conditions, the absorption spectra of Poly A films were systematically determined by FT-IR and subtraction spectroscopy at different temperatures of 25 ± 1 ℃ and relative humidity from 0% to 100% -800cm ~ (-1). According to the changes of the peak position and the intensity of the infrared spectrum, it was confirmed that the hydration of Poly A mainly occurs on the -NH 2 group and the phosphate group in the base. Among them, the spectral intensity of -NH 2 changes obviously when the relative humidity is less than 30%, which is one of the groups with stronger hydration. However, the peak shift of P = 0 is the largest and two vibrational frequencies appear at high humidity, There are two different hydration states. In the hydration process, the peak position of the water peak also has a greater change, and split into multiple peaks.