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目的：应用免疫组化技术SP法结合高压锅抗原修复对36例喉鳞癌P16蛋白的表达进行比较，观察P16蛋白的表述情况。探讨肿瘤抑制基因P16蛋白的表达与临床喉癌病理和吸烟的关系。应用X2检验及精确概率法分析P16蛋白表达与鳞癌分级、浸润深度及转移的相关性。结果：P16蛋白在喉鳞癌中的表达率为63．89％（23／36）；其中，高分化鳞癌P16蛋白的表达率为100％（6／6）；中分化鳞癌P16蛋白的表达率为66．67％（12／18）；低分化鳞癌P16蛋白的表达率为41．67％（5／12），显著低于高中分化组，差异有显著性意义（P＜0．05），结论：P16蛋白的表达与喉鳞癌的分期、浸润深度及转移密切相关。与病人的吸烟程度密切相关。
OBJECTIVE: To compare the expression of P16 protein in 36 specimens of laryngeal squamous cell carcinoma with immunohistochemical SP method and autoclaved antigen retrieval, and to observe the expression of P16 protein. To investigate the relationship between the expression of tumor suppressor gene P16 and clinical pathological changes of laryngeal cancer and smoking. The correlation between the expression of P16 and the grade, depth of invasion and metastasis of squamous cell carcinoma was analyzed by X2 test and exact probability method. Results: The positive rate of P16 protein in laryngeal squamous cell carcinoma was 63.89% (23/36). Among them, the expression rate of P16 protein in well differentiated squamous cell carcinoma was 100% (6/6) The expression rate of P16 protein in poorly differentiated squamous cell carcinoma was 41.67% (5/12), which was significantly lower than that in high differentiated squamous cell carcinoma (P <0.05). 05). Conclusion: The expression of P16 protein is closely related to the staging, depth of invasion and metastasis of LSCC. Closely related to the patient’s smoking level.