一种低渗肿胀试验预测男子生育力

• 作 者: 陈晓星
• 出 处: 国外医学(计划生育分册)
• 发表日期: 1990年03期
• 关键词: 精子膜功能 精液检查 手淫法 前向运动 穿透试验 生育组 精子形态 体外活体染色 体外受精 相差显微镜

低渗肿胀试验(HOS)为一种测定人精子膜功能的简易方法。虽然常规精液检查可以测定精液性质,但不能确定精子功能。目前测定精子功能最常见的是精子穿透试验(SPA),然而SPA费时,实验步骤较复杂,与之相比的HOS为一种测定精子膜完整性及部分精子功能的相对简单的方法。手淫法采集精液,精子活动率(>30%)前向运动(>2.0)和密度(2×10~7/ml)均在正常范围内,只有正常形态的百分率未限定。将精液置于室

Hypotonic swelling test (HOS) is a simple method to determine the function of human sperm membrane. Although routine semen tests determine semen quality, sperm function can not be determined. At present, the sperm penetration test (SPA) is the most common method for determining sperm function. However, the SPA is time-consuming and the experimental procedures are complicated. Compared with HOS, it is a relatively simple method for determining the integrity of sperm membranes and partial sperm function. Semen was collected by masturbation. Sperm motility (> 30%), forward motion (> 2.0) and density (2 × 10 ~ 7 / ml) were all in the normal range, with only the percentage of normal morphology undefined. Put the semen into the room